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insulin like growth factor 1  (R&D Systems)


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    R&D Systems insulin like growth factor 1
    Insulin Like Growth Factor 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+insulin+like+growth+factor+igf/Mouse+IGF-I%2FIGF-1+Antibody/pm25976246-65-53-58
    Average 93 stars, based on 117 article reviews
    insulin like growth factor 1 - by Bioz Stars, 2026-10
    93/100 stars

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    ( A , B ): Assessment of STAT3 signaling, p65 protein abundance, insulin-like growth factor 1 receptor (IGF1-R) abundance, and AKT signaling using total homogenates of kidney medulla ( A ) and cortex ( B ) from male offspring at postnatal day 21 (P21) after perinatal obesity: Immunoblots show phosphorylated STAT3 (pSTAT3) and total STAT3; p65 as an indicator of NFκB signaling; IGF1R protein as well as phosphorylated AKT (pAKT) and total AKT; β-Actin served as loading control. Quantitative densitometric analyses are shown below the immunoblots: quantitative summary displays pSTAT3 relative to total STAT3 or to β-Actin as well as total STAT3 relative to β-Actin; quantification of p65 relative to β-Actin; IGF1R was related to the loading control β-Actin; quantitative summary displays pAKT relative to total AKT or to β-Actin as well as total AKT relative to β-Actin. Standard diet (SD), high-fat diet (HFD, perinatal obesity); n = 6 per group; Mean ± SEM; Mann-Whitney; * p < 0.05; n.s. = not significant.

    Journal: International Journal of Molecular Sciences

    Article Title: Perinatal Obesity Sensitizes for Premature Kidney Aging Signaling

    doi: 10.3390/ijms24032508

    Figure Lengend Snippet: ( A , B ): Assessment of STAT3 signaling, p65 protein abundance, insulin-like growth factor 1 receptor (IGF1-R) abundance, and AKT signaling using total homogenates of kidney medulla ( A ) and cortex ( B ) from male offspring at postnatal day 21 (P21) after perinatal obesity: Immunoblots show phosphorylated STAT3 (pSTAT3) and total STAT3; p65 as an indicator of NFκB signaling; IGF1R protein as well as phosphorylated AKT (pAKT) and total AKT; β-Actin served as loading control. Quantitative densitometric analyses are shown below the immunoblots: quantitative summary displays pSTAT3 relative to total STAT3 or to β-Actin as well as total STAT3 relative to β-Actin; quantification of p65 relative to β-Actin; IGF1R was related to the loading control β-Actin; quantitative summary displays pAKT relative to total AKT or to β-Actin as well as total AKT relative to β-Actin. Standard diet (SD), high-fat diet (HFD, perinatal obesity); n = 6 per group; Mean ± SEM; Mann-Whitney; * p < 0.05; n.s. = not significant.

    Article Snippet: Blots were incubated with the following antibodies: rabbit anti-mouse pAKT (#4058, 1:1000, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse AKT (#9272, 1:2000, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse Insulin-like growth factor receptor 1 (IGF1R) (#3027, 1:1000, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse pSTAT3 (#9145, 1:1000, Cell Signaling, Danvers, MA, USA), mouse anti-mouse STAT3 (#9139, 1:3000, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse p65 (#8242, 1:1000, Cell Signaling, Danvers, MA, USA), and with mouse anti-mouse β-Actin (#3700, 1:10.000, Cell Signaling, Danvers, MA, USA) serving as the loading control.

    Techniques: Quantitative Proteomics, Western Blot, Control, MANN-WHITNEY